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Since most gel electrophoresis procedures result in denaturation of the antigen, only polyclonal and monoclonal antibodies that recognize the denatured form of an antigen can be in Western blot analysis. To study proteins that are expressed at very low levels, it is recommended that immunoprecipitation be followed by immunoblotting for more sensitive detection. Aslo see Tips for the storage of antibodies |
Western Blot操作（专业版）
